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Cloning and Expression
Enzymes
FokI
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FokI
FokI from New England Biolabs
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Citations:
(6)
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Supplier Page from
New England Biolabs for
FokI
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Company Info
Citations
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Product Specs
Item
FokI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0109S
Concentration
4 u/µL
Quantity
1000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
GGATG(9/13)
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (6)
(1)
Exploring the Influence of VDR Genetic Variants TaqI, ApaI, and FokI on COVID-19 Severity and Long-COVID-19 Symptoms
Journal of Personalized Medicine
November 28, 2023
Ghayda’ Alhammadin, Yazun Jarrar, Abdalla Madani, Su-Jun Lee
Biotechnology (Seongnam, Republic of Korea). For the
PCR-RFLP
analysis, the restriction enzymes
FokI
(cat. no.
R0109S
) and
TaqI-v2
(cat. no.
R0149S
) were obtained from
New England Biolabs
(Ipswich, MA, USA);
ApaI
(cat.
(2)
Status of Vitamin D Receptor Gene Polymorphism and 25-Hydroxy Vitamin D Deficiency with Essential Hypertension
Indian Journal of Clinical Biochemistry
July 1, 2022
M. Prasad, D. Rajarajeswari, P. Aruna, K. Ramalingam, R. Viswakumar, Nusrath Fathima, Sandeep Kumar Vishwakarma, Aleem Ahmed Khan
of
PCR
mixture of VDR gene amplicons was subjected to RFLP using 0.4 µL of Fok 1 restriction enzyme (
R0109S
,
NEB
) in a reaction mixture of 25 µL with 2.5 µL reaction buffer and 2.1 µL of molecular biology grade
(3)
Enhanced Detection of Viral RNA Species Using FokI-Assisted Digestion of DNA Duplexes and DNA/RNA Hybrids
Analytical Chemistry
May 10, 2022
Juan R. Tejedor et al.
#B7204), 50 nM (1 pmol) of the duplex or heteroduplex substrate, and either 5 units of the RE
FokI
(
NEB
, #
R0109S
) or 10 units of
BanI
(
NEB
, #
R0118S
) in a final volume of 20 μl. Further details of this experimental
(4)
Joint profiling of histone modifications and transcriptome in single cells from mouse brain
Nature Methods
March 1, 2021
Chenxu Zhu, Yanxiao Zhang, Yang Eric Li, Jacinta Lucero, M. Margarita Behrens, Bing Ren
respectively. Add 2.5 µL 10X
Cutsmart buffer
[
NEB
, M7204S], 1 µL
SbfI-HF
[
NEB
, R3642] and 1 µL
FokI
[
NEB
,
R0109S
] and 3.5 µL H2O to DNA-tube. Add 2 µL 10X
Cutsmart buffer
and 1 µL
NotI-HF
[
NEB
, R3189] to RNA-tube.
(5)
25-hydroxyvitamin D deficiency, exacerbation frequency and human rhinovirus exacerbations in chronic obstructive pulmonary disease
BMC Pulmonary Medicine
December 1, 2012
Jennifer K Quint, Gavin C Donaldson, Nancy Wassef, John R Hurst, Michael Thomas, Jadwiga A Wedzicha
polymorphism (rs2228570), a RFLP analysis was carried out by using the
FokI
restriction endonuclease enzyme (
R0109S
New England BioLabs
). The reaction consisted of; 2μl buffer, 7.5μl dH2O, 0.5μl RE (
FokI
) and 10μl
PCR
(6)
Preparation of Group I Introns for Biochemical Studies and Crystallization Assays by Native Affinity Purification
PLoS ONE
August 27, 2009
Quentin Vicens, Anne R. Gooding, Luis F. Duarte, Robert T. Batey
experiments were transcribed by run-off transcription from a plasmid linearized using the
FokI
enzyme (
NEB
#
R0109S
), as described above, but for 4 h at 37°C and in 10 mL-reaction mixes. The RNAs were ethanol precipitated
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FokI from New England Biolabs
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